Bacterial Identification

Bergey’s Manual

Bacterial Identification Key

Dichotomous Key v1.2

Step 1: Primary Tests

Enter the results for your unknown organism. These first five tests will narrow down the possibilities.

Gram Stain
Catalase
Oxidase
Gelatinase
Glucose Utilization

Step 2: Secondary Tests

Now, let’s refine the results. Please enter the data for the remaining tests. Select “N/A” if you did not perform the test.

Hydrogen Sulfide (H₂S)
Indole
Motility
Lactose Utilization
Mannitol Utilization
Hemolysis

Step 3: Identification Result

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